NEBtools brings New England Biolabs’ most popular web tools to your phone, allowing you to plan your experiments from anywhere. Use Enzyme Finder to select a restriction enzyme by category, recognition sequence, overhang or name to find information on that enzyme. Determine buffer and reaction conditions for experiments requiring two restriction enzymes using the Double Digest Finder. When using either of these tools, look for Time-Saver and HF enzymes for the ultimate in convenience. Use Tm Calculator to calculate optimum annealing temperature for PCR primers when using NEB polymerases and buffers. Use NEBioCalculator to convert DNA or RNA mass to moles, calculate insert ligation amounts, convert OD to concentration, and calculate basic dilution and molarity. NEBtools for Android also enables quick and easy access to NEB Technical Support by phone, email, or web when you need it.
Version 2.4.0 updates the restriction enzyme data used by Enzyme Finder and Double Digest. Minor updates were also made to supplements in digest protocols. This release includes the new naming scheme for NEBuffers which reflects the new BSA-free formulations.